Biotin Conjugation Kit (SpinDesalt Column)
Biotin is a widely used and powerful tool for research due to the specific and high affinity with streptavidin/avidin. Antibody or protein conjugated with several biotin molecules can also amplify the detection signal through streptavidin-conjugated molecule such as streptavidin-HRP, streptavidin-FITC, etc. Biotinylated antibody or protein can be used in various applications including ELISA, WB, IHC, IFA and FACS. Biotin Labeling Kit (SpinDesalt Column) is designed for biotinylation of a small quantity (100 μg-200 μg) of antibody or protein. It provides a rapid and easy process with high efficiency to conjugate biotin to antibody or protein.
Component:
Stability & Storage:
Important notes:
1. Biotin labeling
(1) Antibody concentrations of 1-2 mg/mL generally give optimal results. Recommended amount and volume of antibody for optimal results.
(2) Common non-buffering salts (e.g. sodium chloride) have no effect on conjugation efficiency. Avoid buffer component that contains primary amine
(e.g. amino acid or ethanolamine) and thiols (e.g.2-Mercaptoethanol or DTT).
Components that have on effect or little effect on labeling reaction:
-up to 50 mM Tris
-up to 50 mM HEPES
-up to 10% glycerol
-up to 0.02% sodium azide
2. SpinDesalt Column
(1) For conjugates < 0.1 mL, please use PBS to adjust the volume to at least 0.1 mL to increase the recovery rate of SpinDesalt Column.
(2) The recovery rate of the SpinDesalt Column is related to the type of protein and other biomolecules, usually exceeding 85%. Increasing the sample concentration or volume can improve the recovery rate.
(3) After completing the conjugation, the resin bed in the column can be temporarily stored in PBS.
Shipping Conditions:
Blue ice
| 100 μg x 10 | |
| Biotin | 1 mg, 1 vial |
| Biotin reconstitution buffer | 300 μL, 1 vial |
| 10X Modifier | 300 μL, 1 vial |
| PBS | 10 mL, 1 vial |
| SpinDesalt Column(C08012-K01) | 10 vials |
Stability & Storage:
(1) Stored at -20°C except for SpinDesalt Column stored at 4°C. Avoid repeated freeze/thaw cycles.
(2) Equilibrate kit to room temperature before use.
(3) The kit is stable for one year under proper storage conditions.Important notes:
1. Biotin labeling
(1) Antibody concentrations of 1-2 mg/mL generally give optimal results. Recommended amount and volume of antibody for optimal results.
| Kit size | Antibody amount | Reaction volume |
| 100 μg x 10 | 0.1 mg-2 mg | 100-250 μL |
(e.g. amino acid or ethanolamine) and thiols (e.g.2-Mercaptoethanol or DTT).
Components that have on effect or little effect on labeling reaction:
-up to 50 mM Tris
-up to 50 mM HEPES
-up to 10% glycerol
-up to 0.02% sodium azide
2. SpinDesalt Column
(1) For conjugates < 0.1 mL, please use PBS to adjust the volume to at least 0.1 mL to increase the recovery rate of SpinDesalt Column.
(2) The recovery rate of the SpinDesalt Column is related to the type of protein and other biomolecules, usually exceeding 85%. Increasing the sample concentration or volume can improve the recovery rate.
(3) After completing the conjugation, the resin bed in the column can be temporarily stored in PBS.
Shipping Conditions:
Blue ice
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